Bed bugs have crawled into the cabs of train drivers and caused services to be cancelled as staff worked to clear the affected areas.
The bugs affected the West Marina train care depot near Hastings, East Sussex, which serves routes between London and Kent.
Southeastern trains have been removed from schedules after staff found the insects all over their bags.
Drivers have reportedly refused to continue on board the trains with bugs and have claimed their managers are not taking it seriously.
Trains have been cancelled since they were first spotted last week on September 22 and at least three were removed from schedule on Friday.
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